Journal: Biomolecules and Biomedicine

Article Title: ERBB4 as a therapeutic target in aortic dissection: Implications for cell-based therapies in vascular regeneration

doi: 10.17305/bb.2025.11925

Figure Lengend Snippet: Phenotype transition of VSMC cells. (A) IHC results revealed the decreased levels of α-actin, and TAGLN in aortic tissue; (B) Western blot results presented the decreased levels of α-actin, SPP1, TAGLN, and the highly expressed of epiregulin in aortic tissue. Bar graphs presented the quantification of α-actin (C), SPP1 (D), TAGLN (E), and epiregulin (F). vs Control group, ** P < 0.01, *** P < 0.001. VSMC: Vascular smooth muscle cell; IHC: Immunohistochemical; TAGLN: Transgelin.

Article Snippet: To prevent non-specific binding, membranes were blocked with 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBST) for 1 h at room temperature, followed by overnight incubation at 4 ∘ C with primary antibodies against ERBB4 (1:1000, Proteintech, Wuhan, China), α-actin (1:1000, Proteintech), Transgelin (TAGLN) (1:1000, Elabsience, Shanghai, China), SPP1 (1:1000, ZENBIO, Chengdu, China), epiregulin (1:1000, Biorbyt, UK), CD151 (1:1000, Proteintech), ITGAE (1:1000, ab224202, Abcam, UK), ITGB5 (1:1000, Proteintech), p-IκBα (1:5000, Proteintech), IκBα (1:5000, Proteintech), p-NF-κB-65 (1:2000, Proteintech), NF-κB-65 (1:1000, Proteintech), and GAPDH (1:50000, Proteintech) as a loading control.

Techniques: Western Blot, Control, Immunohistochemical staining

Journal: Endocrinology

Article Title: Manganese-induced Precocious Puberty Alters Mammary Epithelial Cell Proliferation in Female Rats

doi: 10.1210/endocr/bqaf052

Figure Lengend Snippet: Effect of MnPP on PR-associated protein expression. Representative western blots (A and B) show expression of PR-B, EREG, P27, and SP1 in epithelial cell lysates from controls (lanes 1-3) and MnPP (lanes 4-6) animals at PND 30 and PND 120, respectively. Bar graphs show changes in the mean (±SEM) of respective protein expression levels for PR-B (C), EREG (D), Sp1 (E), and p27 (F), at PND 30 (left) and PND 120 (right). Animal numbers (N) are shown within the bars. Data was analyzed by Student t -test. * P < .05; ** P < .01.

Article Snippet: Western blot analysis was performed as previously described (Dearth et al, 2014), using anti-Sp1 (1:500, Sigma-Aldrich Cat# 07-645, RRID:AB_310773 ), anti-p27 (1:500, (Cell Signaling Technology Cat# 3686, RRID:AB_2077850 ), anti-c-myc (1:500, Cell Signaling Technology Cat# 5605, RRID:AB_1903938 ), anti-amphiregulin (AREG) (1:750, Bioss Cat# bs-3847R, RRID:AB_10886273 ), anti-epiregulin (EREG) (1:500, Santa Cruz Biotechnology Cat# sc-376284, RRID:AB_11014189 ), and anti-HNF-3α/FoxA1 (FOXA1) (3 μg/μL, Novus Cat# NBP2-45269, RRID:AB_2687601 and anti-PR (1:500, Thermo Fisher Scientific Cat# MA1-410, RRID:AB_2164327 ).

Techniques: Expressing, Western Blot